Part:BBa_K2989006
Ce-HBsAg-95
Competitive endogenous RNA (ceRNA) hypothesis describes a complex post-transcriptional regulatory network that includes lncRNA, mRNA, and other types of RNA. They act as natural miRNA sponges that inhibit the function of other RNA by competing for one or more miRNA binding sites.
Usage
Ce-HBsAg-95 is located in the downstream region of the hulc promoter. After specific initiation in tumor cells, the downstream Ce-HBsAg-95 is expressed. Ce-HBsAg-95 can competingly inhibit miR-HBsAg-95 to allow transcription of HBsAg It, together with miR-HBsAg-95, helps to form a regulation system.
A: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-95
B: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-95 co-transformed with pCDNA3.1(+)-Hulc-CeR-95
We transfected plasmids into HepG2 cells: in Figure A, we transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-95 alone while in Figure B, we co-transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-95 and pCDNA3.1(+)-Hulc-CeR-95 plasmid. The figures showed that the EmGFP brightness slightly increased after we co-transfected the CeR-95 part, which showed that the CeR-95 component didn’t bind well for the corresponding miRNA, but it still worked.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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